Error: Feature names of counts matrix cannot be empty

[mcsintonn]

Moved this to issues

Hi,

I'm brand new to single cell and I'm trying to use Seurat for the first time. I have a CellRanger output, with a raw_feature_bc_matrix folder. This folder contains three compressed files: barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz.

I have read this folder into R Studio using the following line, as recommended in the Seurat guide:

naive1.data <- Read10X(data.dir = "/Users/Desktop/naive_raw_feature_bc_matrix")

I then try to use the CreateSeuratObject command as follows:

adipnaive <- CreateSeuratObject(counts = naive1.data, project = "TrypWAT", min.cells = 3, min.features = 200)

At this point, I get the following error:
Error: Feature names of counts matrix cannot be empty

I'm completely lost here, and was wondering if anyone has any insights into what might be causing the problem? I was wondering if it might be because as well as mouse genes, there are also trypanosome genes in this file. I have attached an example of the file (although in Excel format rather than .tsv)
features_file_example.xlsx

Any advice would be very much appreciated!

[howtofindme]

Moved this to issues

Hi,

I'm brand new to single cell and I'm trying to use Seurat for the first time. I have a CellRanger output, with a raw_feature_bc_matrix folder. This folder contains three compressed files: barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz.

I have read this folder into R Studio using the following line, as recommended in the Seurat guide:

naive1.data <- Read10X(data.dir = "/Users/Desktop/naive_raw_feature_bc_matrix")

I then try to use the CreateSeuratObject command as follows:

adipnaive <- CreateSeuratObject(counts = naive1.data, project = "TrypWAT", min.cells = 3, min.features = 200)

At this point, I get the following error: Error: Feature names of counts matrix cannot be empty

I'm completely lost here, and was wondering if anyone has any insights into what might be causing the problem? I was wondering if it might be because as well as mouse genes, there are also trypanosome genes in this file. I have attached an example of the file (although in Excel format rather than .tsv) features_file_example.xlsx

Any advice would be very much appreciated!

same problem ,have you solved it?

[qingmemng]

Hi
I meet the same problem?