unable to read and incorporate the Sample information from molecule_info.h5 to seurat object.

[Shamzar]

Hello,
I quite new to Single cell analysis and I am running into the following problem. I had 12 different samples sequenced on Illumina, 6 of them are control and other 6 are treatment. I ran cellranger --count function on all 12 samples together and got outs folder with following files.

analysis
filtered_feature_bc_matrix
metrics_summary.csv
possorted_genome_bam.bam
raw_feature_bc_matrix
web_summary.html
cloupe.cloupe h5
molecule_info.h5
possorted_genome_bam.bam.bai
raw_feature_bc_matrix.h5

I used the raw_feature_bc_matrix.h5 file to generate seurat object however its missing the sample information. In order to put sample information in this seurat object I am trying to read molecule_info.h5 file and pull the sample information from it and put that into the Seurat object@meta.data.

Although, I am unable to read this molecule_info.h5 using Read10Xh5 command because it keeps giving me error.

metadata_dir <- "/home/Single_cell/yard/1013MJ1/outs/molecule_info.h5"
Mh5 <- Read10X_h5(metadata_dir)
Error in x$exists(name) : HDF5-API Errors:
error #000: H5L.c in H5Lexists(): line 845: unable to get link info
class: HDF5
major: Links
minor: Can't get value

error #001: H5L.c in H5L__exists(): line 2932: path doesn't exist
    class: HDF5
    major: Links
    minor: Object already exists

error #002: H5Gtraverse.c in H5G_traverse(): line 848: internal path traversal failed
    class: HDF5
    major: Symbol table
    minor: Object not found

error #003: H5Gtraverse.c in H5G__traverse_real(): line 579: can't look up component
    class: HDF5
    major: Symbol table
    minor: Object not found

error #004: H5Gobj.c in H5G__obj_lookup(): line 1135: can't locate object
    class: HDF5
    major: Symbol table
    minor: Object not found

error #005: H5Gstab.c in H5G__stab_lookup(): line 876: can't read message
    class: HDF5
    major: Symbol table
    minor: Unrecognized message

error #006: H

Can you help me get Sample information incorporated into my seurat object. Thankyou!