ScaleData Killed- Is my scRNA file too large?

[kostberg21]

Hi all,

I'm reaching out here because I can't find any documentation on the ScaleData function failing on a Seurat object. I've been following this tutorial: https://satijalab.org/seurat/reference/findmarkers to generate cluster graphs for my 10x single cell data. I have aggregated 4 samples into one dataset for comparison purposes. The code runs smoothly up until the below function. Then it is killed. How do I fix this? Any help would be appreciated!

all.genes <- rownames(object)
object <- ScaleData(object, features = all.genes)
Centering and scaling data matrix
Killed.

My file size is listed here:
barcodes.tsv.gz = 191K
features.tsv.gz = 326K
matrix.mtx.gz = 310M