MGI single cell rna-seq as input of seurat

[Zoeyau531]

Dear All,

I would like to know if I can input MGI single cell rna-seq data into Seurat for analysis?

I only used 10X data for seurat before.

What will be the "filtered_feature_bc_matrix" which includes barcodes.tsv.gz, features.tsv.gz and matrix.mtx.gz in MGI dnbc4tools output?

thanks!

[wudustan]

You can create a Seurat object from any kind of matrix that is structured as features x cells.

mgi.seurat <- CreateSeuratObject(mgi.counts, assay = "RNA")

It looks like MGI have given some basic guidance on how to work with common single cell analysis software here