Error when loading data withLoad10X_Spatial:Unable to read the PNG file

[aaa070707]

Hello!
I encountered an issue while using the "Load10X_Spatial" function from Seurat to load spatial transcriptomics data.Here are the detailed error messages and the troubleshooting process:

localdir <- "D:/Visium_HD_Mouse_Brain_binned_outputs"
object <- Load10X_Spatial(data.dir = localdir, bin.size = c(8, 16))
Error in png::readPNG(source = file.path(image.dir, image.name)) :
file is not in PNG format

The structure of my data directory is as follows:
我的数据目录结构如下：
D:/Visium_HD_Mouse_Brain_binned_outputs
├── binned_outputs/
├── square_002um/
│ ├── square_008um/
│ │ └── spatial/
│ │ ├── tissue_lowres_image.png
│ │ ├── tissue_positions_list.csv
│ │ ├── tissue_positions.parquet
│ │ ├── scalefactors_json.json
│ │ ├── filtered_feature_bc_matrix.h5
│ └── square_016um/
│ └── spatial/
│ │ ├── tissue_lowres_image.png
│ │ ├── tissue_positions_list.csv
│ │ ├── tissue_positions.parquet
│ │ ├── scalefactors_json.json
│ │ ├── filtered_feature_bc_matrix.h5

Initially,I found that thespatial/tissue_lowres_image.pngfile existed and was located at the correct path,but it was corrupted and could not be opened properly.I downloaded the Visium_HD_Mouse_Brain_spatial.tar.gz from the 10x Genomics official website and replaced the corrupted PNG file with a valid one from the same source.However,the error persisted.

Environment information:
• Seurat version:Seurat v5.2.0
• R version:R 4.3.3
• Operating System:Windows 10

Despite trying various methods to fix the PNG file,I still could not resolve the error withLoad10X_Spatial.I suspect the issue might be due to one of the following reasons:
• Seurat has specific requirements for the PNG file format,and the repaired file does not meet these requirements.
• There might be issues with the data directory structure or file naming.
I am providing the following files for further analysis:
• The repaired PNG file

Thank you very much for your help!If there is any further information needed,please feel free to contact me at any time.

[a-mathewson]

Hello! Have you been able to resolve this issue? I'm running in to the same error with my own dataset.