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I have a problem with PCA assay step in Seurat (RunPCA() function)
When I use cellranger count matrix at my laptop computer R studio, I could get the proper PCA plot and I could do further steps,
But, When I use same matrix file at my laboratory server computer (by connecting R studio server), I got wrong PCA plot.
I attached the image files of them.
The Seurat version is same, only R version is different between those two computers (4.4.3 in server, 4.4.2 in my laptop)
And I check that all commands were same.
My laptop computer has lower memory performance, and I need to handle more big data, so I must use my server computer,
but that kind of error let me keep out of using our lab server computer.
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I have a problem with PCA assay step in Seurat (RunPCA() function)
When I use cellranger count matrix at my laptop computer R studio, I could get the proper PCA plot and I could do further steps,
But, When I use same matrix file at my laboratory server computer (by connecting R studio server), I got wrong PCA plot.
I attached the image files of them.
The Seurat version is same, only R version is different between those two computers (4.4.3 in server, 4.4.2 in my laptop)
And I check that all commands were same.
My laptop computer has lower memory performance, and I need to handle more big data, so I must use my server computer,
but that kind of error let me keep out of using our lab server computer.
Please help me,,,!!
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