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My major concern is that when these 2,489 unscaled features were excluded from PCA, the resulting dimensionality reduction and clustering produced abnormal results - cells appear poorly separated/mixed together. |
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After running SCTransform followed by RunPCA, I encountered this warning:
Warning: The following 2489 features requested have not been scaled (running reduction without them)This aligns with multiple reports #8880 that the issue occurs when scale.data contains significantly fewer features than var.features.
A potential fix is reducing
min_cellsto retain more features in scaling.Is this adjustment (
min_cellsreduction) a recommended practice in the community?Could this introduce any negative impacts (e.g., noise amplification, batch effect sensitivity)?
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