@@ -9,3 +9,53 @@ mass spectrometry-based proteomics data using **msDiaLogue** in R.
99Each recipe focuses on one module of the analysis workflow, allowing users
1010to easily combine recipes into workflows that best fit their own analyses.
1111
12+
13+ ## What is msDiaLogue?
14+
15+
16+ ** msDiaLogue** is an R package developed through a collaboration between
17+ the [ Proteomics & Metabolomics Facility (PMF)] ( https://proteomics.uconn.edu/ )
18+ and the [ Statistical Consulting Services (SCS)] ( https://statsconsulting.uconn.edu/ )
19+ at the [ University of Connecticut (UConn)] ( https://uconn.edu/ )
20+ It provides a modular workflow for analyzing untargeted mass spectrometry
21+ (MS)-based proteomics data, together with a wide range of visualization options
22+ for both Data-Dependent Acquisition (DDA) and Data-Independent Acquisition (DIA)
23+ datasets.
24+
25+
26+ ::: {style="text-align: center;"}
27+ [ ![ UConn] ( https://raw.githubusercontent.com/uconn-scs/msDiaLogue/main/man/figures/uconn.png ) {style="width:34%; margin:0 2%"}] ( https://uconn.edu/ )
28+ [ ![ CORE] ( https://raw.githubusercontent.com/uconn-scs/msDiaLogue/refs/heads/main/man/figures/core.png ) {style="width:30.6%; margin:0 2%"}] ( https://core.uconn.edu/ )
29+ [ ![ SCS] ( https://raw.githubusercontent.com/uconn-scs/msDiaLogue/refs/heads/main/man/figures/scs.png ) {style="width:25.5%; margin:0 0%"}] ( https://statsconsulting.uconn.edu/ )
30+ :::
31+
32+
33+ ## Design philosophy
34+
35+
36+ Unlike monolithic analysis pipelines, ** msDiaLogue** is designed as a modular
37+ workflow.
38+ Each analysis step is implemented as an independent module that can be used on
39+ its own or combined with other modules.
40+ This design allows users to adapt the workflow to different experimental designs,
41+ software platforms, and analysis objectives.
42+
43+
44+ ## Workflow
45+
46+
47+ :::: {style="text-align: center;"}
48+ ![ ] ( https://raw.githubusercontent.com/uconn-scs/msDiaLogue/refs/heads/main/man/figures/workflow.png )
49+ ::::
50+
51+
52+ ## Organization of this cookbook
53+
54+
55+ The cookbook is organized into independent recipes corresponding to the major
56+ modules of ** msDiaLogue** .
57+ Each chapter introduces one module using practical examples and can be read
58+ independently.
59+ Readers may follow the recipes in sequence as a complete workflow or use
60+ individual chapters as references for specific analysis tasks.
61+
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