Masato

Masato is a modular amplicon sequencing analysis tookit. It highlights transparent and composible command line interface that helps users assemble and understand their pipeline.

NOTE: This package is renamed from easy_amplicon. The old repository has been archived.

Try in Colab

Thie Colab notebook demonstrates basic usage of the package, example data included.

Preprocessing

Primers

• 16SV4

  Fwd (2+19bp)
      GTGTGYCAGCMGCCGCGGTAA
      └┘└─────────────────┘
    Linker  16SV4-515F-Y
  
  Rev (2+20bp)
      CCGGACTACNVGGGTWTCTAAT
      └┘└──────────────────┘
    Linker  16SV4-515R-B
  

• 16SV3V4

• 16SV4V5

A note about the fesibility of pair merging