Multichannel Igor Electrophysiology Suite

The Multichannel Igor Electrophysiology Suite (MIES) is a proven sweep based data acquisition software package for intracellular electrophysiology (patch clamp) [1, 2, 3, 4, 5, 6]. It offers top of its class flexibility and robustness for stimulus generation, data acquisition, and analysis.

Highlights

• Run up to five DACs in parallel for scalable data acquisition.
• Acquire data on up to eight headstage on a single DAC to probe up to 56 connections at once.
• Create arbitrarily complex stimulus sets with an intuitive GUI.
• Export all data, including all of its metadata, into the industry-standard NWBv2-format and read it back in
• Run custom code during data acquisition for Automatic Experiment Control and real-time adaptability.
• Automate experimental setup using configuration files for streamlined workflows.
• Interact with MIES from other programming languages (Python, C++, Javascript, ...) through ZeroMQ.
• Track and manage your experiments with comprehensive metadata acquisition and browsing tools.
• Leverage the integrated scripting language for flexible, on-the-fly electrophysiology data evaluation.
• Ensure compatibility with every earlier version of MIES, protecting your previous work and workflows.

Getting started

• Download the latest release
• Windows: Run the installer
• MacOSX (analysis only): Manual installation
• View the documentation

Brief visual overview

Postsynaptic data analysis

Video tutorial of the postsynaptic potential/postsynaptic current data (PSX) analysis module

Required Software

• Igor Pro 9 (nightly) or later

For Data Analysis

• Windows 10 64-bit or MacOSX 10.10

For Data Acquisition

• Windows 10 64-bit
• NIDAQ MX XOP

Supported Hardware

• Digital to analog converters (DAC):

  • National Instruments:

    • PCIe-6341

    • PCIe-6343

    • PXI-6259

    • USB-6346

      Other NI hardware models can be added on request. Please open an issue from within MIES, MIES Panels->Report an issue, for that.

  • Instrutech/HEKA ITC:

    • 16
    • 18
    • 1600

    ITC devices are at the End-Of-Service-Life (EOSL). National Instruments DACs are recommended for MIES users looking for new hardware.

• Amplifier: Molecular Devices 700B

• Pressure control (optional):

  • QPV High Resolution Pressure Regulator from Proportion-Air, Inc.; Part name: QPV regulator (custom, see part No), -10 psi to +10 psi; Part no.: QPV1TBNEEN10P10PSGAXLDD
  • controlled through ITC 18/1600 or National Instruments USB 6001

Support statement

The last released version receives fixes for all critical bugs.

Bug reporting

Report a Bug