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6th & 20th June 2025, Francis Crick Institute

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Overview

In this workshop, we will bridge the gap between advanced microscopy data generation and the computational skills required for its analysis. By leveraging open-source tools like FIJI and Jupyter, participants will learn to automate image analysis, enhancing the precision, efficiency, and reproducibility of their research. This two-day event, led by experienced core facility staff from the Francis Crick Institute, offers a practical approach to mastering quantitative analysis and workflow automation, essential for advancing research across multiple domains.

Registration

You can register your interest in attending the workshop here. Please be advised that places are limited and a selection process will be used in the event that the workshop is over-subscribed.

The deadline for registering your interest is May 9th.

Instructors

Preparation

  1. Please remember to bring your laptop (and charger).
  2. Please install the required software before the workshop - follow the installation instructions on this page.
  3. Download the workshop data by clicking on the link to the ZIP archive at the top of this page.
  4. You will be assigned to a specific group, with whom you will be sitting - your group number will be displayed in the training room.
  5. PLEASE CONTACT US BEFORE THE WORKSHOP IF YOU ENCOUNTER ANY DIFFICULTIES WITH ANY OF THE ABOVE.

Slides

All of the slides for this workshop are available here.

Program

Friday, June 6th 2025
12:00 - 14:30 Session 1

Image Pre-Processing, Segmentation & Analysis

Dave Barry

  • Practical Applications:
    • Basic segmentation using thresholding
    • Use of filtering to suppress noise
    • Obtaining numbers from images
14:30 - 14:50 Coffee Break
14:50 - 17:00 Session 2

Assembling Pipelines and Interpreting Results

Dave Barry

  • Practical Applications:
    • Counting and quantifying morphology of objects
    • Quantifying fluorescence intensities
Friday, June 20th 2025
12:00 - 13:30 Session 3

Introduction to Python and installations

Alicja Skórkowska

  • Creating Python environments
  • Installing Jupyter notebooks and napari
13:30 - 13:50 Coffee Break
13:50 - 16:00 Session 4

Using Jupyter Notebooks for Reproducible Analysis

Alicja Skórkowska

  • Variables and arrays
  • Practical Application: quantify morphology of objects in a 2D image with Jupyter
16:00 - 17:00 Session 5 Q&A and Discussion

Venue

The workshop will take place in Training Room 1 at the Francis Crick Institute.

image

Previous Workshops

Date Venue Content
8 / 9th April 2024 King's College London Click here
24 / 25th April 2024 Royal College of Surgeons in Ireland Click here
21 / 22nd October 2024 Francis Crick Institute Click here
8 / 9th April 2025 University of Galway, Ireland Click here

FAQ

  1. Do I need any prior knowledge of image analysis, FIJI or napari to attend?

    No, this workshop is aimed at complete beginners, but a basic understanding of image acquisition would be beneficial.

  2. Do I need to have any experience of coding?

    While some basic knowledge would be helpful, it's not essential and even if you have no knowledge of python, Jupyter notebooks or FIJI scripts/macros, you should still apply.

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  • Jupyter Notebook 86.5%
  • ImageJ Macro 13.3%
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