Added enzymatic tissue dissociation fields

docs/jsonBrowser/type.md

@@ -190,7 +190,11 @@ schema_type | The type of the metadata schema entity. | string | yes |  |  | pro
 provenance | Provenance information provided by the system. | object | no | [See   provenance](.md#provenance) |  |  | 
 protocol_core | Core protocol-level information. | object | yes | [See core  protocol_core](core.md#protocol-core) | Protocol core |  | 
 method | How cells or organelles were dissociated. | object | yes | [See module  process_type_ontology](module.md#process-type-ontology) | Dissociation method |  | 
-reagents | A list of purchased reagents used in this protocol. | array | no | [See module  purchased_reagents](module.md#purchased-reagents) | Protocol reagents |  | 
+reagents | A list of purchased reagents used in this protocol. | array | no | [See module  purchased_reagents](module.md#purchased-reagents) | Purchased reagents |  | 
+digestion_time | Time of digestion of the specimen. | integer | no |  | Digestion time |  | 2; 5; 10
+digestion_time_unit | The unit in which the digestion time is expressed. | object | no | [See module  time_unit_ontology](module.md#time-unit-ontology) | Digestion time unit |  | 
+digestion_temperature | Temperature of digestion in Celsius or in general terms (i.e. cold, warm, room temperature). | string | no |  | Digestion temperature |  | 4; 37; cold
+digestion_solution | Enzyme(s) or reagent(s) of the solution that was used for the digestion of the specimen. | array | no |  | Digestion solution | Citric-acid based, Detergent-based, Lysis buffer, Collagenase, Collagenase A, Collagenase B, Collagenase complex, Collagenase D, Collagenase I, Collagenase II, Collagenase IV, Dispase, Dispase II, DNAse, DNAse I, Elastase, Liberase, Protease, Trypsin, Trypsin-like | 
 
 ## iPSC induction protocol
 _Information about how a biomaterial is treated to become an induced pluripotent stem cell._

json_schema/type/protocol/biomaterial_collection/dissociation_protocol.json

@@ -54,7 +54,56 @@
                 "type": "object",
                 "$ref": "module/process/purchased_reagents.json"
             },
-            "user_friendly": "Protocol reagents"
+            "user_friendly": "Purchased reagents"
+        },
+        "digestion_time": {
+            "description": "Time of digestion of the specimen.",
+            "type": "integer",
+            "example": "2; 5; 10",
+            "user_friendly": "Digestion time"
+        },
+        "digestion_time_unit": {
+            "description": "The unit in which the digestion time is expressed.",
+            "type": "object",
+            "$ref": "module/ontology/time_unit_ontology.json",
+            "user_friendly": "Digestion time unit"
+        },
+        "digestion_temperature": {
+            "description": "Temperature of digestion in Celsius or in general terms (i.e. cold, warm, room temperature).",
+            "pattern": "\\d+(\\.\\d+)?|frozen|cold|room temperature|warm",
+            "type": "string",
+            "example": "4; 37; cold",
+            "user_friendly": "Digestion temperature"
+        },
+        "digestion_solution": {
+            "description": "Enzyme(s) or reagent(s) of the solution that was used for the digestion of the specimen.",
+            "type": "array",
+            "items": {
+                "type": "string",
+                "enum": [
+                    "Citric-acid based",
+                    "Detergent-based",
+                    "Lysis buffer",
+                    "Collagenase",
+                    "Collagenase A",
+                    "Collagenase B",
+                    "Collagenase complex",
+                    "Collagenase D",
+                    "Collagenase I",
+                    "Collagenase II",
+                    "Collagenase IV",
+                    "Dispase",
+                    "Dispase II",
+                    "DNAse",
+                    "DNAse I",
+                    "Elastase",
+                    "Liberase",
+                    "Protease",
+                    "Trypsin",
+                    "Trypsin-like"
+                        ]
+            },
+            "user_friendly": "Digestion solution"
         }
     }
 }

json_schema/update_log.csv

@@ -1 +1,6 @@
 Schema,Change type,Change message,Version,Date
+type/protocol/biomaterial_collection/dissociation_protocol,minor,Changed of user_friendly name of reagent field,,
+type/protocol/biomaterial_collection/dissociation_protocol,minor,Added optional digestion_time field,,
+type/protocol/biomaterial_collection/dissociation_protocol,minor,Added optional digestion_time_unit field,,
+type/protocol/biomaterial_collection/dissociation_protocol,minor,Added optional digestion_temperature field,,
+type/protocol/biomaterial_collection/dissociation_protocol,minor,Added optional digestion_solution field,,