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[aperkins19]

OPTIMISATIONS

• map tips to plate - the same tips are used for each sample well in the the wash steps ✔️
• Mapped sample rna to pcr plate transfer (and added pcr plate) ✔️
• blow air over beads when drying✔️
• Tuned for alcohol viscosities. ✔️
• Make sure first dispense is from top of well so not to waste pipette or contaminate ✔️

NOTE

• There is one major, unresolved problem that I've identified:

  • I've mapped a designated tip box to be used for the ethanol washes and mapped it to the sample plate.

  • The tips are returned between washes and reused.

  • However:

  • When supernatant is being trashed, the pipette has to make multiple trips. e.g. 400ul = 2x 200ul trips.

  • When the m300 is ejecting the liquid, the end tip closest to the front of the deck, touches the trash bucket (where it is slanted) and is contaminated.

  • I think it would be easiest to solve with a hard coded move_to( #coordinates within trash). I've writted trash_coordinates_V2 doing just that. Trial it tomorrow and tune accordingly

YOU NEED TO INCLUDE THE LABWARE FILES IN A FOLDER CALLED 'labware' IN YOUR DIRECTORY if you want to simulate
message me on the slack if you have any problems.

the files are already loaded on to the A09 OT2 so that is ready to go.

[aperkins19]

My optimisations.

please refer to the comments at the start of rna_extraction_jupyter_V5.ipynb

if you want to simulate: create a folder called labware and put the Nunc and Fischer .json files inside.