OpentronDev

Opentron Setup and Protocol Developments

• Task: Set-Up of 3 OpenTrons stations and qPCR machines + inventory what is missing?
• Location: Open Cell; Contact: Helene at +447400277072

Station A: Sample Plating (needs to be in a separate room)

• p20 + p300 single-channel pipettes
• Temperature Module (actively cool control RNA)
• 4x tube racks
• 96 Deepwell Extraction Plate
• 1x rack 20ul + 1x rack 200ul filter tips

Station B: RNA Extraction (potentially needs to be placed in a BSL cabinet)

• p300 8-channel pipette
• Magnetic Module
• 96 Deepwell Extraction Plate
• 96 Well Elution Plate
• 5x racks of 200ul filter tips
• 2x 12-channel reagent reservoirs
• 2x 1-channel liquid trash reservoirs

Station C - Assay Setup (next to qPCR machine) - extra clean environment!

• p20 single-channel
• Opentrons Temperature Module
• 96 Well Elution Plate
• 96 Well PCR Plate
• 3x racks 20ul filter tips

Link to set-up
OpenTrons Blog: testing-for-covid-19-with-opentrons

Useful links:

• Inventory list for the set-up Google sheet
• Action Sequence Google doc