Mass spectrometry-based metabolomics introduction

The course will introduce MS-based metabolomics data, it's basic processing and the downstream analysis. The course will be a mix of lectures and hands-on session where we will perform the basic processing steps using metaboigniter, a nextflow workflow, and acore, short for analytical core for the downstream analysis.

Location

DTU Campus - Building 421

Auditorium A071 from 9.00 to 12.15

• Introduction with overview of metabolomics and preprocessing
• Steps in data processing and running metaboigniter (hands-on)

Auditorium A073 from 12.15 to 16.30

• Lunch
• Hands-on
  • Data cleaning, normalization and statistical analysis
  • Annotation and Interpretation

Agenda

Time	Topic	lecturer
09.00 - 09.10	Welcome, coffee, and setup	-
09.10 - 10.30	Introduction with overview of metabolomics and preprocessing	Maria Barranco-Altirriba
10.30 - 10.45	break	-
10.45 - 12.15	Steps in data processing and running metaboigniter (hands-on)	Henry Webel
12.15 - 13.00	Lunch (sandwiches are provided)	-
13.00 - 13.45	Data cleaning, normalization and statistical analysis (lecture)	Felicia Schulz
13.45 - 13.55	break	-
13.55 - 14.40	Data cleaning, normalization and statistical analysis (Hands-On)	Felicia Schulz
14.40 - 14.50	break	-
14.50 - 15.35	Annotation and Interpretation (lecture)	Maria Barranco-Altirriba
15.35 - 15.45	break	-
15.45 - 16.30	Annotation and Interpretation (hands-On)	Henry Webel

Some details to the agenda points

09.10-10.30 - Introduction with overview of metabolomics and preprocessing

See the slides for introduction: link

10.45-12.15 - Steps in data processing and running metaboigniter

Find the instruction here and the slides.

13.00 - 14.40 - Data cleaning, normalization and statistical analysis

See the slides for the statistical analysis: will be here

14.50 - 16.30 - Data cleaning, normalization and statistical analysis

Find the notebook here and the slides.