GSE274058 reference run (A100 confirmed)

GSE274058 reference run

Authoritative A100 rerun for the shen_2026_scrnaseq (GSE274058) dissociated reference track.

• Runtime: Python 3.10.12 on Linux A100 host.
• Dataset scale: 41,749 cells, 32,317 genes, 5 samples.
• Barcode status: 707 single, 285 multiple, 40,757 unassigned cells.
• Inference set: 17 valid non-control perturbations, all completed with method="pseudobulk".
• A100 comparison status: replaced; core dataset metrics and Lrrk2/Srf top-hit signatures matched the Windows draft, while program matrices were regenerated from the A100 run and now retain top-50 coverage for every successful perturbation.

Biological interpretation: use this as a reference-side transcriptional signature package, not as strong target-knockdown evidence. Only 6 of 17 valid perturbations show target-gene log2fc < 0; Lrrk2 and Srf do not show convincing self-knockdown in this run.

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