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Add nf-test: test_alternativepreprocessing#695

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jfy133 merged 28 commits intodevfrom
nftest-alternativepreprocessing
Mar 19, 2026
Merged

Add nf-test: test_alternativepreprocessing#695
jfy133 merged 28 commits intodevfrom
nftest-alternativepreprocessing

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@jfy133 jfy133 commented Mar 1, 2026

Directory structure to check:

results_altpreprocessing/
├── adapterremoval
│   ├── 2612_ERR5766176_B.settings
│   ├── 2612_ERR5766176.settings
│   └── 2613_ERR5766181.settings
├── bbduk
│   ├── 2612_ERR5766176_B.bbduk.log
│   ├── 2612_ERR5766176.bbduk.log
│   └── 2613_ERR5766181.bbduk.log
├── bowtie2
│   └── align
│       ├── 2612_ERR5766176_B.bowtie2.log
│       ├── 2612_ERR5766176.bowtie2.log
│       └── 2613_ERR5766181.bowtie2.log
├── fastqc
│   ├── processed
│   │   ├── 2612_ERR5766176_B_processed_1_fastqc.html
│   │   ├── 2612_ERR5766176_B_processed_1_fastqc.zip
│   │   ├── 2612_ERR5766176_B_processed_2_fastqc.html
│   │   ├── 2612_ERR5766176_B_processed_2_fastqc.zip
│   │   ├── 2612_ERR5766176_processed_1_fastqc.html
│   │   ├── 2612_ERR5766176_processed_1_fastqc.zip
│   │   ├── 2612_ERR5766176_processed_2_fastqc.html
│   │   ├── 2612_ERR5766176_processed_2_fastqc.zip
│   │   ├── 2613_ERR5766181_processed_1_fastqc.html
│   │   ├── 2613_ERR5766181_processed_1_fastqc.zip
│   │   ├── 2613_ERR5766181_processed_2_fastqc.html
│   │   ├── 2613_ERR5766181_processed_2_fastqc.zip
│   │   ├── ERR3201952_ERR3201952_processed_fastqc.html
│   │   └── ERR3201952_ERR3201952_processed_fastqc.zip
│   └── raw
│       ├── 2612_ERR5766176_B_raw_1_fastqc.html
│       ├── 2612_ERR5766176_B_raw_1_fastqc.zip
│       ├── 2612_ERR5766176_B_raw_2_fastqc.html
│       ├── 2612_ERR5766176_B_raw_2_fastqc.zip
│       ├── 2612_ERR5766176_raw_1_fastqc.html
│       ├── 2612_ERR5766176_raw_1_fastqc.zip
│       ├── 2612_ERR5766176_raw_2_fastqc.html
│       ├── 2612_ERR5766176_raw_2_fastqc.zip
│       ├── 2613_ERR5766181_raw_1_fastqc.html
│       ├── 2613_ERR5766181_raw_1_fastqc.zip
│       ├── 2613_ERR5766181_raw_2_fastqc.html
│       ├── 2613_ERR5766181_raw_2_fastqc.zip
│       ├── ERR3201952_ERR3201952_raw_fastqc.html
│       └── ERR3201952_ERR3201952_raw_fastqc.zip
├── filtlong
│   └── ERR3201952_ERR3201952_filtered.log
├── kaiju
│   ├── db5
│   │   ├── 2611_db5.kaijutable.txt
│   │   ├── 2611_db5.kaiju.tsv
│   │   ├── 2612_db5.kaijutable.txt
│   │   ├── 2612_db5.kaiju.tsv
│   │   ├── 2613_db5.kaijutable.txt
│   │   └── 2613_db5.kaiju.tsv
│   ├── db6
│   │   ├── 2614_db6.kaijutable.txt
│   │   ├── 2614_db6.kaiju.tsv
│   │   ├── ERR3201952_db6.kaijutable.txt
│   │   └── ERR3201952_db6.kaiju.tsv
│   └── db7
│       ├── 2611_db7.kaijutable.txt
│       ├── 2611_db7.kaiju.tsv
│       ├── 2612_db7.kaijutable.txt
│       ├── 2612_db7.kaiju.tsv
│       ├── 2613_db7.kaijutable.txt
│       ├── 2613_db7.kaiju.tsv
│       ├── 2614_db7.kaijutable.txt
│       ├── 2614_db7.kaiju.tsv
│       ├── ERR3201952_db7.kaijutable.txt
│       └── ERR3201952_db7.kaiju.tsv
├── kraken2
│   └── db2
│       ├── 2611_db2.kraken2.kraken2.report.txt
│       ├── 2612_db2.kraken2.kraken2.report.txt
│       └── 2613_db2.kraken2.kraken2.report.txt
├── multiqc
│   ├── multiqc_data
│   │   ├── ar_length_count_plot_All.txt
│   │   ├── ar_length_count_plot_Discarded.txt
│   │   ├── ar_length_count_plot_Mate1.txt
│   │   ├── ar_length_count_plot_Mate2.txt
│   │   ├── ar_length_count_plot_Singleton.txt
│   │   ├── ar_retained_plot.txt
│   │   ├── bbduk-filtered-barplot_Bases.txt
│   │   ├── bbduk-filtered-barplot_Reads.txt
│   │   ├── bbduk.txt
│   │   ├── bowtie2_pe_plot.txt
│   │   ├── fastqc-1_adapter_content_plot.txt
│   │   ├── fastqc-1_per_base_n_content_plot.txt
│   │   ├── fastqc-1_per_base_sequence_quality_plot.txt
│   │   ├── fastqc-1_per_sequence_gc_content_plot_Counts.txt
│   │   ├── fastqc-1_per_sequence_gc_content_plot_Percentages.txt
│   │   ├── fastqc-1_per_sequence_quality_scores_plot.txt
│   │   ├── fastqc-1_sequence_counts_plot.txt
│   │   ├── fastqc-1_sequence_duplication_levels_plot.txt
│   │   ├── fastqc-1-status-check-heatmap.txt
│   │   ├── fastqc_adapter_content_plot.txt
│   │   ├── fastqc_per_base_n_content_plot.txt
│   │   ├── fastqc_per_base_sequence_quality_plot.txt
│   │   ├── fastqc_per_sequence_gc_content_plot_Counts.txt
│   │   ├── fastqc_per_sequence_gc_content_plot_Percentages.txt
│   │   ├── fastqc_per_sequence_quality_scores_plot.txt
│   │   ├── fastqc_sequence_counts_plot.txt
│   │   ├── fastqc_sequence_duplication_levels_plot.txt
│   │   ├── fastqc_sequence_length_distribution_plot.txt
│   │   ├── fastqc-status-check-heatmap.txt
│   │   ├── filtlong.txt
│   │   ├── kaiju-topfive-plot.txt
│   │   ├── kraken-top-n-plot_Class.txt
│   │   ├── kraken-top-n-plot_Domain.txt
│   │   ├── kraken-top-n-plot_Family.txt
│   │   ├── kraken-top-n-plot_Genus.txt
│   │   ├── kraken-top-n-plot_Kingdom.txt
│   │   ├── kraken-top-n-plot_Order.txt
│   │   ├── kraken-top-n-plot_Phylum.txt
│   │   ├── kraken-top-n-plot_Species.txt
│   │   ├── kraken-top-n-plot_Unclassified.txt
│   │   ├── llms-full.txt
│   │   ├── multiqc_adapter_removal.txt
│   │   ├── multiqc_bowtie2.txt
│   │   ├── multiqc_citations.txt
│   │   ├── multiqc_data.json
│   │   ├── multiqc_fastqc_fastqc-1.txt
│   │   ├── multiqc_fastqc.txt
│   │   ├── multiqc_general_stats.txt
│   │   ├── multiqc_kaiju_species.txt
│   │   ├── multiqc_kraken.txt
│   │   ├── multiqc.log
│   │   ├── multiqc.parquet
│   │   ├── multiqc_samtools_stats.txt
│   │   ├── multiqc_software_versions.txt
│   │   ├── multiqc_sources.txt
│   │   ├── porechop-endtrim-barplot.txt
│   │   ├── porechop-middlesplit-barplot.txt
│   │   ├── porechop_porechop.txt
│   │   ├── porechop-starttrim-barplot.txt
│   │   ├── samtools_alignment_plot.txt
│   │   └── samtools-stats-dp.txt
│   ├── multiqc_plots
│   │   ├── pdf
│   │   │   ├── ar_length_count_plot_All.pdf
│   │   │   ├── ar_length_count_plot_Discarded.pdf
│   │   │   ├── ar_length_count_plot_Mate1.pdf
│   │   │   ├── ar_length_count_plot_Mate2.pdf
│   │   │   ├── ar_length_count_plot_Singleton.pdf
│   │   │   ├── ar_retained_plot-cnt.pdf
│   │   │   ├── ar_retained_plot-pct.pdf
│   │   │   ├── bbduk-filtered-barplot_Bases-cnt.pdf
│   │   │   ├── bbduk-filtered-barplot_Bases-pct.pdf
│   │   │   ├── bbduk-filtered-barplot_Reads-cnt.pdf
│   │   │   ├── bbduk-filtered-barplot_Reads-pct.pdf
│   │   │   ├── bowtie2_pe_plot-cnt.pdf
│   │   │   ├── bowtie2_pe_plot-pct.pdf
│   │   │   ├── fastqc-1_adapter_content_plot.pdf
│   │   │   ├── fastqc-1_per_base_n_content_plot.pdf
│   │   │   ├── fastqc-1_per_base_sequence_quality_plot.pdf
│   │   │   ├── fastqc-1_per_sequence_gc_content_plot_Counts.pdf
│   │   │   ├── fastqc-1_per_sequence_gc_content_plot_Percentages.pdf
│   │   │   ├── fastqc-1_per_sequence_quality_scores_plot.pdf
│   │   │   ├── fastqc-1_sequence_counts_plot-cnt.pdf
│   │   │   ├── fastqc-1_sequence_counts_plot-pct.pdf
│   │   │   ├── fastqc-1_sequence_duplication_levels_plot.pdf
│   │   │   ├── fastqc_adapter_content_plot.pdf
│   │   │   ├── fastqc_per_base_n_content_plot.pdf
│   │   │   ├── fastqc_per_base_sequence_quality_plot.pdf
│   │   │   ├── fastqc_per_sequence_gc_content_plot_Counts.pdf
│   │   │   ├── fastqc_per_sequence_gc_content_plot_Percentages.pdf
│   │   │   ├── fastqc_per_sequence_quality_scores_plot.pdf
│   │   │   ├── fastqc_sequence_counts_plot-cnt.pdf
│   │   │   ├── fastqc_sequence_counts_plot-pct.pdf
│   │   │   ├── fastqc_sequence_duplication_levels_plot.pdf
│   │   │   ├── fastqc_sequence_length_distribution_plot.pdf
│   │   │   ├── kaiju-topfive-plot-cnt.pdf
│   │   │   ├── kaiju-topfive-plot-pct.pdf
│   │   │   ├── kraken-top-n-plot_Class-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Class-pct.pdf
│   │   │   ├── kraken-top-n-plot_Domain-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Domain-pct.pdf
│   │   │   ├── kraken-top-n-plot_Family-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Family-pct.pdf
│   │   │   ├── kraken-top-n-plot_Genus-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Genus-pct.pdf
│   │   │   ├── kraken-top-n-plot_Kingdom-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Kingdom-pct.pdf
│   │   │   ├── kraken-top-n-plot_Order-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Order-pct.pdf
│   │   │   ├── kraken-top-n-plot_Phylum-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Phylum-pct.pdf
│   │   │   ├── kraken-top-n-plot_Species-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Species-pct.pdf
│   │   │   ├── kraken-top-n-plot_Unclassified-cnt.pdf
│   │   │   ├── kraken-top-n-plot_Unclassified-pct.pdf
│   │   │   ├── porechop-endtrim-barplot-cnt.pdf
│   │   │   ├── porechop-endtrim-barplot-pct.pdf
│   │   │   ├── porechop-middlesplit-barplot-cnt.pdf
│   │   │   ├── porechop-middlesplit-barplot-pct.pdf
│   │   │   ├── porechop-starttrim-barplot-cnt.pdf
│   │   │   ├── porechop-starttrim-barplot-pct.pdf
│   │   │   ├── samtools_alignment_plot-cnt.pdf
│   │   │   ├── samtools_alignment_plot-pct.pdf
│   │   │   └── samtools-stats-dp.pdf
│   │   ├── png
│   │   │   ├── ar_length_count_plot_All.png
│   │   │   ├── ar_length_count_plot_Discarded.png
│   │   │   ├── ar_length_count_plot_Mate1.png
│   │   │   ├── ar_length_count_plot_Mate2.png
│   │   │   ├── ar_length_count_plot_Singleton.png
│   │   │   ├── ar_retained_plot-cnt.png
│   │   │   ├── ar_retained_plot-pct.png
│   │   │   ├── bbduk-filtered-barplot_Bases-cnt.png
│   │   │   ├── bbduk-filtered-barplot_Bases-pct.png
│   │   │   ├── bbduk-filtered-barplot_Reads-cnt.png
│   │   │   ├── bbduk-filtered-barplot_Reads-pct.png
│   │   │   ├── bowtie2_pe_plot-cnt.png
│   │   │   ├── bowtie2_pe_plot-pct.png
│   │   │   ├── fastqc-1_adapter_content_plot.png
│   │   │   ├── fastqc-1_per_base_n_content_plot.png
│   │   │   ├── fastqc-1_per_base_sequence_quality_plot.png
│   │   │   ├── fastqc-1_per_sequence_gc_content_plot_Counts.png
│   │   │   ├── fastqc-1_per_sequence_gc_content_plot_Percentages.png
│   │   │   ├── fastqc-1_per_sequence_quality_scores_plot.png
│   │   │   ├── fastqc-1_sequence_counts_plot-cnt.png
│   │   │   ├── fastqc-1_sequence_counts_plot-pct.png
│   │   │   ├── fastqc-1_sequence_duplication_levels_plot.png
│   │   │   ├── fastqc_adapter_content_plot.png
│   │   │   ├── fastqc_per_base_n_content_plot.png
│   │   │   ├── fastqc_per_base_sequence_quality_plot.png
│   │   │   ├── fastqc_per_sequence_gc_content_plot_Counts.png
│   │   │   ├── fastqc_per_sequence_gc_content_plot_Percentages.png
│   │   │   ├── fastqc_per_sequence_quality_scores_plot.png
│   │   │   ├── fastqc_sequence_counts_plot-cnt.png
│   │   │   ├── fastqc_sequence_counts_plot-pct.png
│   │   │   ├── fastqc_sequence_duplication_levels_plot.png
│   │   │   ├── fastqc_sequence_length_distribution_plot.png
│   │   │   ├── kaiju-topfive-plot-cnt.png
│   │   │   ├── kaiju-topfive-plot-pct.png
│   │   │   ├── kraken-top-n-plot_Class-cnt.png
│   │   │   ├── kraken-top-n-plot_Class-pct.png
│   │   │   ├── kraken-top-n-plot_Domain-cnt.png
│   │   │   ├── kraken-top-n-plot_Domain-pct.png
│   │   │   ├── kraken-top-n-plot_Family-cnt.png
│   │   │   ├── kraken-top-n-plot_Family-pct.png
│   │   │   ├── kraken-top-n-plot_Genus-cnt.png
│   │   │   ├── kraken-top-n-plot_Genus-pct.png
│   │   │   ├── kraken-top-n-plot_Kingdom-cnt.png
│   │   │   ├── kraken-top-n-plot_Kingdom-pct.png
│   │   │   ├── kraken-top-n-plot_Order-cnt.png
│   │   │   ├── kraken-top-n-plot_Order-pct.png
│   │   │   ├── kraken-top-n-plot_Phylum-cnt.png
│   │   │   ├── kraken-top-n-plot_Phylum-pct.png
│   │   │   ├── kraken-top-n-plot_Species-cnt.png
│   │   │   ├── kraken-top-n-plot_Species-pct.png
│   │   │   ├── kraken-top-n-plot_Unclassified-cnt.png
│   │   │   ├── kraken-top-n-plot_Unclassified-pct.png
│   │   │   ├── porechop-endtrim-barplot-cnt.png
│   │   │   ├── porechop-endtrim-barplot-pct.png
│   │   │   ├── porechop-middlesplit-barplot-cnt.png
│   │   │   ├── porechop-middlesplit-barplot-pct.png
│   │   │   ├── porechop-starttrim-barplot-cnt.png
│   │   │   ├── porechop-starttrim-barplot-pct.png
│   │   │   ├── samtools_alignment_plot-cnt.png
│   │   │   ├── samtools_alignment_plot-pct.png
│   │   │   └── samtools-stats-dp.png
│   │   └── svg
│   │       ├── ar_length_count_plot_All.svg
│   │       ├── ar_length_count_plot_Discarded.svg
│   │       ├── ar_length_count_plot_Mate1.svg
│   │       ├── ar_length_count_plot_Mate2.svg
│   │       ├── ar_length_count_plot_Singleton.svg
│   │       ├── ar_retained_plot-cnt.svg
│   │       ├── ar_retained_plot-pct.svg
│   │       ├── bbduk-filtered-barplot_Bases-cnt.svg
│   │       ├── bbduk-filtered-barplot_Bases-pct.svg
│   │       ├── bbduk-filtered-barplot_Reads-cnt.svg
│   │       ├── bbduk-filtered-barplot_Reads-pct.svg
│   │       ├── bowtie2_pe_plot-cnt.svg
│   │       ├── bowtie2_pe_plot-pct.svg
│   │       ├── fastqc-1_adapter_content_plot.svg
│   │       ├── fastqc-1_per_base_n_content_plot.svg
│   │       ├── fastqc-1_per_base_sequence_quality_plot.svg
│   │       ├── fastqc-1_per_sequence_gc_content_plot_Counts.svg
│   │       ├── fastqc-1_per_sequence_gc_content_plot_Percentages.svg
│   │       ├── fastqc-1_per_sequence_quality_scores_plot.svg
│   │       ├── fastqc-1_sequence_counts_plot-cnt.svg
│   │       ├── fastqc-1_sequence_counts_plot-pct.svg
│   │       ├── fastqc-1_sequence_duplication_levels_plot.svg
│   │       ├── fastqc_adapter_content_plot.svg
│   │       ├── fastqc_per_base_n_content_plot.svg
│   │       ├── fastqc_per_base_sequence_quality_plot.svg
│   │       ├── fastqc_per_sequence_gc_content_plot_Counts.svg
│   │       ├── fastqc_per_sequence_gc_content_plot_Percentages.svg
│   │       ├── fastqc_per_sequence_quality_scores_plot.svg
│   │       ├── fastqc_sequence_counts_plot-cnt.svg
│   │       ├── fastqc_sequence_counts_plot-pct.svg
│   │       ├── fastqc_sequence_duplication_levels_plot.svg
│   │       ├── fastqc_sequence_length_distribution_plot.svg
│   │       ├── kaiju-topfive-plot-cnt.svg
│   │       ├── kaiju-topfive-plot-pct.svg
│   │       ├── kraken-top-n-plot_Class-cnt.svg
│   │       ├── kraken-top-n-plot_Class-pct.svg
│   │       ├── kraken-top-n-plot_Domain-cnt.svg
│   │       ├── kraken-top-n-plot_Domain-pct.svg
│   │       ├── kraken-top-n-plot_Family-cnt.svg
│   │       ├── kraken-top-n-plot_Family-pct.svg
│   │       ├── kraken-top-n-plot_Genus-cnt.svg
│   │       ├── kraken-top-n-plot_Genus-pct.svg
│   │       ├── kraken-top-n-plot_Kingdom-cnt.svg
│   │       ├── kraken-top-n-plot_Kingdom-pct.svg
│   │       ├── kraken-top-n-plot_Order-cnt.svg
│   │       ├── kraken-top-n-plot_Order-pct.svg
│   │       ├── kraken-top-n-plot_Phylum-cnt.svg
│   │       ├── kraken-top-n-plot_Phylum-pct.svg
│   │       ├── kraken-top-n-plot_Species-cnt.svg
│   │       ├── kraken-top-n-plot_Species-pct.svg
│   │       ├── kraken-top-n-plot_Unclassified-cnt.svg
│   │       ├── kraken-top-n-plot_Unclassified-pct.svg
│   │       ├── porechop-endtrim-barplot-cnt.svg
│   │       ├── porechop-endtrim-barplot-pct.svg
│   │       ├── porechop-middlesplit-barplot-cnt.svg
│   │       ├── porechop-middlesplit-barplot-pct.svg
│   │       ├── porechop-starttrim-barplot-cnt.svg
│   │       ├── porechop-starttrim-barplot-pct.svg
│   │       ├── samtools_alignment_plot-cnt.svg
│   │       ├── samtools_alignment_plot-pct.svg
│   │       └── samtools-stats-dp.svg
│   └── multiqc_report.html
├── pipeline_info
│   ├── execution_report_2026-03-01_13-15-35.html
│   ├── execution_report_2026-03-01_13-24-00.html
│   ├── execution_report_2026-03-01_13-26-31.html
│   ├── execution_report_2026-03-01_13-28-50.html
│   ├── execution_report_2026-03-01_13-32-27.html
│   ├── execution_report_2026-03-01_13-35-51.html
│   ├── execution_report_2026-03-01_13-38-01.html
│   ├── execution_report_2026-03-01_13-40-19.html
│   ├── execution_report_2026-03-01_13-40-55.html
│   ├── execution_timeline_2026-03-01_13-15-35.html
│   ├── execution_timeline_2026-03-01_13-24-00.html
│   ├── execution_timeline_2026-03-01_13-26-31.html
│   ├── execution_timeline_2026-03-01_13-28-50.html
│   ├── execution_timeline_2026-03-01_13-32-27.html
│   ├── execution_timeline_2026-03-01_13-35-51.html
│   ├── execution_timeline_2026-03-01_13-38-01.html
│   ├── execution_timeline_2026-03-01_13-40-19.html
│   ├── execution_timeline_2026-03-01_13-40-55.html
│   ├── execution_trace_2026-03-01_13-15-35.txt
│   ├── execution_trace_2026-03-01_13-24-00.txt
│   ├── execution_trace_2026-03-01_13-26-31.txt
│   ├── execution_trace_2026-03-01_13-28-50.txt
│   ├── execution_trace_2026-03-01_13-32-27.txt
│   ├── execution_trace_2026-03-01_13-35-51.txt
│   ├── execution_trace_2026-03-01_13-38-01.txt
│   ├── execution_trace_2026-03-01_13-40-19.txt
│   ├── execution_trace_2026-03-01_13-40-55.txt
│   ├── nf_core_taxprofiler_software_mqc_versions.yml
│   ├── params_2026-03-01_13-15-46.json
│   ├── params_2026-03-01_13-24-11.json
│   ├── params_2026-03-01_13-26-42.json
│   ├── params_2026-03-01_13-29-01.json
│   ├── params_2026-03-01_13-32-37.json
│   ├── params_2026-03-01_13-36-01.json
│   ├── params_2026-03-01_13-38-12.json
│   ├── params_2026-03-01_13-40-31.json
│   ├── params_2026-03-01_13-41-04.json
│   ├── pipeline_dag_2026-03-01_13-15-35.html
│   ├── pipeline_dag_2026-03-01_13-24-00.html
│   ├── pipeline_dag_2026-03-01_13-26-31.html
│   ├── pipeline_dag_2026-03-01_13-28-50.html
│   ├── pipeline_dag_2026-03-01_13-32-27.html
│   ├── pipeline_dag_2026-03-01_13-35-51.html
│   ├── pipeline_dag_2026-03-01_13-38-01.html
│   ├── pipeline_dag_2026-03-01_13-40-19.html
│   └── pipeline_dag_2026-03-01_13-40-55.html
├── porechop
│   └── ERR3201952_ERR3201952_porechop.log
└── samtools
    └── stats
        ├── 2612_ERR5766176_B.stats
        ├── 2612_ERR5766176.stats
        ├── 2613_ERR5766181.stats
        └── ERR3201952_ERR3201952.stats

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/taxprofiler branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core pipelines lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

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github-actions bot commented Mar 1, 2026

This PR is against the master branch ❌

  • Do not close this PR
  • Click Edit and change the base to dev
  • This CI test will remain failed until you push a new commit

Hi @jfy133,

It looks like this pull-request is has been made against the nf-core/taxprofiler master branch.
The master branch on nf-core repositories should always contain code from the latest release.
Because of this, PRs to master are only allowed if they come from the nf-core/taxprofiler dev branch.

You do not need to close this PR, you can change the target branch to dev by clicking the "Edit" button at the top of this page.
Note that even after this, the test will continue to show as failing until you push a new commit.

Thanks again for your contribution!

@github-actions github-actions bot added the size/m label Mar 1, 2026
@jfy133 jfy133 changed the title Nftest alternativepreprocessing Add nf-test: test_alternativepreprocessing Mar 1, 2026
@jfy133 jfy133 changed the base branch from master to dev March 1, 2026 13:52
@jfy133 jfy133 marked this pull request as draft March 1, 2026 13:52
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github-actions bot commented Mar 1, 2026

nf-core pipelines lint overall result: Passed ✅ ⚠️

Posted for pipeline commit 80f0f62

+| ✅ 314 tests passed       |+
#| ❔   1 tests were ignored |#
!| ❗  18 tests had warnings |!
Details

❗ Test warnings:

  • files_exist - File not found: conf/igenomes.config
  • files_exist - File not found: conf/igenomes_ignored.config
  • pipeline_todos - TODO string in nextflow.config: Specify any additional parameters here
  • local_component_structure - kraken2_standard_report.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
  • local_component_structure - krona_cleanup.nf in modules/local should be moved to a TOOL/SUBTOOL/main.nf structure
  • local_component_structure - shortread_preprocessing.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - nonpareil.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - longread_adapterremoval.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - longread_filtering.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - shortread_fastp.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - visualization_krona.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - longread_preprocessing.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - shortread_adapterremoval.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - shortread_hostremoval.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - longread_hostremoval.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - profiling.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - standardisation_profiles.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure
  • local_component_structure - shortread_complexityfiltering.nf in subworkflows/local should be moved to a SUBWORKFLOW_NAME/main.nf structure

❔ Tests ignored:

  • files_unchanged - File ignored due to lint config: .github/PULL_REQUEST_TEMPLATE.md

✅ Tests passed:

Run details

  • nf-core/tools version 3.5.2
  • Run at 2026-03-19 09:30:33

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jfy133 commented Mar 1, 2026

Unstable files to address:

  • bbduk.log
  • fastqc.zip
  • kaiju.tsv
  • porchop.log

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jfy133 commented Mar 1, 2026

@nf-core-bot fix linting

@github-actions github-actions bot added the size/s label Mar 1, 2026
@github-actions github-actions bot added the size/l label Mar 1, 2026
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jfy133 commented Mar 3, 2026

@nf-core-bot fix linting

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jfy133 commented Mar 4, 2026

@nf-core-bot fix linting

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jfy133 commented Mar 4, 2026

@nf-core-bot fix linting

@jfy133 jfy133 marked this pull request as ready for review March 4, 2026 11:28
@jfy133 jfy133 requested a review from sofstam March 5, 2026 04:08
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jfy133 commented Mar 18, 2026

I'll do a review before I merge that I'm still following the same structure as the two more recent PRs such as #700

@jfy133 jfy133 marked this pull request as draft March 19, 2026 04:54
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jfy133 commented Mar 19, 2026

TODO: update config/snapshot to use prinseqplusplus instead for shortread complexity filtering

@jfy133 jfy133 marked this pull request as ready for review March 19, 2026 09:29
@jfy133 jfy133 requested a review from sofstam March 19, 2026 09:30
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There is a md5 difference in sylph combined report. This will be adressed in the PR I will open soon

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Regarding the inconsistency in the sylph combined report, I would also add the file here, right?

@jfy133 jfy133 merged commit 9c4734f into dev Mar 19, 2026
16 of 18 checks passed
@jfy133 jfy133 deleted the nftest-alternativepreprocessing branch March 19, 2026 12:10
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4 participants