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MadridJan2014Tomography

Adrian Quintana edited this page Dec 11, 2017 · 1 revision

Hands-on Training school on soft X-ray cryo-tomography reconstruction of cells.

$ LOCATION: Residencia de Estudiantes CSIC (Madrid. Spain). $ DATES: January 15th-17th, 2014

NEWS:

  • Registration is closed
  • Slideshows are online. You can download the slideshows from the talks (PDF). Just click on the link of each talk (see #Programme_and_resources below)

## Outline of the training school

X-ray imaging is a new tool in structural biology. Right now two synchrotron beamlines in Europe (!U41-TXM at HZB-BESSY II in Berlin and Mistral at ALBA in Barcelona) are devoted to soft X-ray cryo-tomography of cells, allowing structural biologists to use soft X-ray microscopy. BESSY II beamline started users operation in 2009 and Mistral just opened in 2012, therefore the community making use of these facilities is still small, although continuously growing due to the increasing interest in this technique. New users are not always familiar with tomography and the available image processing software packages for dealing with their data. This training school will strengthen the image capacities of new researchers so that relevant scientific information can be extracted from their experimental data.

The aim of this training schools is bringing together experts and users at a starting level for a practical session on how to deal with the data collected at the synchrotrons, covering from pre-processing to post-processing: normalization, alignment, reconstruction, filtering and segmentation. An introduction to tomography and to image formation with soft X-ray lenses (i.e. Fresnel Zone Plates) will be included to give an overview of the specific problems and limitations that have to be faced with this technique.

Registration

The course fee is 350E, which includes accommodation and all meals during the course.

Additionally, there are 6 travel grants for attendees from COST countries (http://www.cost.eu/domains_actions/mpns/Actions/MP1207?parties), for up to 550E, to cover airplane tickets (tourist class only) and accomodation. Their Registration fee will be covered byBiostruct-X project.

If you want to apply for a travel grant, please send an e-mail to [email protected] including:

  • short CV
  • brief explanation of the impact of this course in your work

Registration fee for those living in Madrid: 220 euro.

Deadline for registration: December 1st, 2013.

Registration is closed. A participant list will appear on December 5th.

Payment information

Bank transfer to: Centro Nacional de Biotecnología - CNB
Bank Name: Santander Central Hispano
Address: Julio Palacios, 23. 28029, Madrid. Spain
Account: 0049 5926 41 2816155571
IBAN: ES91 0049 5926 4128 1615 5571
Swift: BSCHESMM

Please include your name in the wire concept

Requirements

Please bring a laptop, or alternatively pair up with another particpant who has a laptop. Wireless connection will come handy.

Participants

A participant list will appear on December 5th.

Name Institute
Tanja Ducic CELLS - ALBA
Esteban Veiga cnb-csic
Jasminka Boskovic CNIO
Sathish Yadav University of Groningen
Marie-Charlotte Domart Cancer Research UK, London Research Institute
Raffaella Carzaniga London Research Institute/ CRUK
Charlotte Melia Leiden University Medical Center
Dilip Kumar CSIR-IGIB
Kaiming Zhang Peking university
Anastasia Gazi CNRS
Hind AL-Khayat Qatar Foundation
Michele Darrow National Center for Macromolecular Imaging at Baylor College of Medicine
Andrea Sorrentino ALBA synchrotron
Xeniya Grafskaya
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Ramirez Santiago Guillermo CNB-CSIC
Anna Laromaine Sagué ICMAB-CSIC
Nebraska Katherine Zambrano Pineda
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Ana J Perez Berna ALBA
Io Almagro Universidad de Granada
Hardt Martin University of Giessen
Ruben Cardenes Universitat Pompeu Fabra

Programme and resources

The training school starts on January Wednesday 15, 2014 at 13:30. The agenda is as follows:

Wednesday 15th

13:30 - Welcome Reception

14:30 - Registration

15:00 - J.L. Carrascosa: /JLC-20140116-intro_microscopy_cellular_context.pdf

16:30 - COFFEE BREAK

17:00 - Attendees: 5 minutes talks

Thursday 16th

09:00 - Eva Pereiro: /EP-20140116-Soft_Xray.pdf

11:00 - COFFEE BREAK

11:30 - "Alignment & Reconstruction"

13:30 - LUNCH

14:30 - Practical session I

16:30 - COFFEE BREAK

17:00 - Practical session II

Friday 17th

09:00 - Denoising & Segmentation (Tips&Tricks)

11:00 - COFFEE BREAK

11:30 - Practical session III

13:30 - LUNCH

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