PCR related articles and other detection techniques

COVID-19

A public repository of scientific and media resources of SARS-CoV-2 related COVID-19 maintained by ITER.

---

PCR related articles and other detection techniques

Subsection dedicated to PCR (RT-qPCR) and related topics.

A Confirmed Case of SARS-CoV-2 Pneumonia with Routine RT-PCR Negative and Virus Variation in Guangzhou, China

A handheld point-of-care system for rapid detection of SARS-CoV-2 in under 20 minutes

A protocol for massively parallel diagnosis and genome sequencing of SARS-CoV-

A rapid, low cost, and highly sensitive SARS-CoV-2 diagnostic based on whole genome sequencing

A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material

A simple colorimetric molecular detection of novel coronavirus (COVID-19), an essential diagnostic tool for pandemic screening

A streamlined approach to rapidly detect SARS-CoV-2 infection, avoiding RNA extraction

An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage

Amplicon based MinION sequencing of SARS-CoV-2 and metagenomic characterisation of nasopharyngeal swabs from patients with COVID-19

Analysis of factors associated early diagnosis in coronavirus disease 2019 (COVID-19)

Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow

Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysis

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification (ai-LAMP) for Rapid and Reliable Detection of SARS-CoV-2

BEARmix: Basic Economical Amplification Reaction one-step RT-qPCR master mix

Combination of RT‐qPCR testing and clinical features for diagnosis of COVID‐19 facilitates management of SARS‐CoV‐2 outbreak

Comparing library preparation methods for SARS-CoV-2 multiplex amplicon sequencing on the Illumina MiSeq platform

Comparison of Four Molecular In Vitro Diagnostic Assays for the Detection of SARS-CoV-2 in Nasopharyngeal Specimens

Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes

Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes

COVID-19 Protocolo - Grupo de investigación Neuronal Ubiquitin Pathways - UPV/EHU

CRISPR–Cas12-based detection of SARS-CoV-2

Database of SARS-CoV2-relevant control materials: Specs, Vendors, and more.

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19)

Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

Detection of Respiratory Viruses in Sputum from Adults by Use of Automated Multiplex PCR

Developing antibody tests for SARS-CoV-2

DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP

Efficient high throughput SARS-CoV-2 testing to detect asymptomatic carriers

Estimating false-negative detection rate of SARS-CoV-2 by RT-PCR

Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus

Evaluation of WHO listed COVID-19 qPCR primers and probe in silico with 375 SERS-CoV-2 full genome sequences

Failure of the cobas® SARS-CoV-2 (Roche) E-gene assay is associated with a C-to-T transition at position 26340 of the SARS-CoV-2 genome

FALSE-NEGATIVE RESULTS OF INITIAL RT-PCR ASSAYS FOR COVID-19: A SYSTEMATIC REVIEW

False Negative Tests for SARS-CoV-2 Infection — Challenges and Implications

Fast and accurate diagnostics from highly multiplexed sequencing assays

Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples

Fast SARS-CoV-2 detection protocol based on RNA precipitation and RT-qPCR in nasopharyngeal swab samples

FDA Posts New Template for At-Home and Over-the-Counter Diagnostic Tests for Use in Non-Lab Settings, Such as Homes, Offices or Schools

Genotyping SARS-CoV-2 through an interactive web application

High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads

In silico analysis of RT-qPCR designs recommended by WHO for detection of SARS-CoV-2 and a commercial kit validated following UNE/EN ISO 17025:2005 and two reference laboratories

LamPORE, for rapid, highly scalable, low-cost COVID-19 detection

Large-scale, in-house production of viral transport media to support SARS-CoV-2 PCR testing in a multi-hospital healthcare network during the COVID-19 pandemic

Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens

Molecular detection of SARS-CoV-2 using a reagent-free approach

Multiplex Isothermal Amplification Coupled with Nanopore Sequencing for Rapid Detection and Mutation Surveillance of SARS-CoV-2

Non-specific Primers Reveal False-negative Risk in Detection of COVID-19 Infections

Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction

Organisms causing secondary pneumonias in COVID-19 patients at 5 UK ICUs as detected with the FilmArray test

Overcoming the bottleneck to widespread testing: A rapid review of nucleid acid testing approaches for COVID-19 detection

Performance of the rapid Nucleic Acid Amplification by Abbott ID NOW COVID-19 in nasopharyngeal swabs transported in viral media and dry nasal swabs, in a New York City academic institution

Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection

Pooled RNA sample reverse transcriptase real time PCR assay for SARS CoV-2 infection: a reliable, faster and economical method

Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-Co2 virus detection

Positive RT-PCR Test Results in Patients Recovered From COVID-19

Preliminary support for a 'dry swab, extraction free' protocol for SARS-CoV-2 testing via RT-qPCR

Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples

Rapid colorimetric detection of COVID-19 coronavirus using a reverse tran-scriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic plat-form: iLACO

Rapid diagnosis of SARS-CoV-2 infection by detecting IgG and IgM antibodies with an immunochromatographic device: a prospective single-center study

Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler

Rapid implementation of real-time SARS-CoV-2 sequencing to investigate healthcare-associated COVID-19 infections

Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP

Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)

Real-time colorimetric LAMP methodology for quantitative nucleic acids detection at the point-of-care

Reconstructed diagnostic sensitivity and specificity of the RT-PCR test for COVID-19

Repeat COVID-19 Molecular Testing: Correlation with Recovery of Infectious Virus, Molecular Assay Cycle Thresholds, and Analytical Sensitivity

Report from the American Society for Microbiology COVID-19 International Summit, 23 March 2020: Value of Diagnostic Testing for SARS–CoV-2/COVID-19

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol

RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swab using Qiagen RNEasy kits or directly via omission of an extraction step

Saliva offers a sensitive, specific and non-invasive alternative to upper respiratory swabs for SARS-CoV-2 diagnosis

Saliva Sample as a Non-Invasive Specimen for the Diagnosis of Coronavirus Disease-2019 (COVID-19): a Cross-Sectional Study

Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs

SARS-CoV-2 detection with CRISPR diagnostics

SARS-CoV-2 Detection Using an Isothermal Amplification Reaction and a Rapid, Inexpensive Protocol for Sample Inactivation and PurificationSmart Pooled sample Testing for COVID-19: A Possible Solution for Sparsity of Test Kits

Screening for SARS-CoV-2 infections with colorimetric RT-LAMP and LAMP sequencing

Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection

Swabs Collected by Patients or Health Care Workers for SARS-CoV-2 Testing

The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step

Variation in False Negative Rate of RT-PCR Based SARS-CoV-2 Tests by Time Since Exposure