PCR related articles and other detection techniques

COVID-19

A public repository of scientific and media resources of SARS-CoV-2 related COVID-19 maintained by ITER.

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PCR related articles and other detection techniques

Subsection dedicated to PCR (RT-qPCR) and related topics.

A protocol for massively parallel diagnosis and genome sequencing of SARS-CoV-

A rapid, low cost, and highly sensitive SARS-CoV-2 diagnostic based on whole genome sequencing

A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material

A simple colorimetric molecular detection of novel coronavirus (COVID-19), an essential diagnostic tool for pandemic screening

A streamlined approach to rapidly detect SARS-CoV-2 infection, avoiding RNA extraction

An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage

Analysis of factors associated early diagnosis in coronavirus disease 2019 (COVID-19)

Analytical sensibility and specificity of two RT-qPCR protocols for SARS-CoV-2 detection performed in an automated workflow

Combination of RT‐qPCR testing and clinical features for diagnosis of COVID‐19 facilitates management of SARS‐CoV‐2 outbreak

Comparison of Four Molecular In Vitro Diagnostic Assays for the Detection of SARS-CoV-2 in Nasopharyngeal Specimens

Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes

Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes

COVID-19 Protocolo - Grupo de investigación Neuronal Ubiquitin Pathways - UPV/EHU

CRISPR–Cas12-based detection of SARS-CoV-2

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19)

Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

Detection of Respiratory Viruses in Sputum from Adults by Use of Automated Multiplex PCR

Developing antibody tests for SARS-CoV-2

DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP

Efficient and Practical Sample Pooling High-Throughput PCR Diagnosis of COVID-19

Estimating false-negative detection rate of SARS-CoV-2 by RT-PCR

Evaluation of WHO listed COVID-19 qPCR primers and probe in silico with 375 SERS-CoV-2 full genome sequences

FALSE-NEGATIVE RESULTS OF INITIAL RT-PCR ASSAYS FOR COVID-19: A SYSTEMATIC REVIEW

Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples

Fast SARS-CoV-2 detection protocol based on RNA precipitation and RT-qPCR in nasopharyngeal swab samples

High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads

Increased PCR screening capacity using a multi-replicate pooling scheme

In silico analysis of RT-qPCR designs recommended by WHO for detection of SARS-CoV-2 and a commercial kit validated following UNE/EN ISO 17025:2005 and two reference laboratories

Large-scale, in-house production of viral transport media to support SARS-CoV-2 PCR testing in a multi-hospital healthcare network during the COVID-19 pandemic

Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens

Non-specific Primers Reveal False-negative Risk in Detection of COVID-19 Infections

Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction

Overcoming the bottleneck to widespread testing: A rapid review of nucleid acid testing approaches for COVID-19 detection

Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection

Pooled RNA sample reverse transcriptase real time PCR assay for SARS CoV-2 infection: a reliable, faster and economical method

Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-Co2 virus detection

Positive RT-PCR Test Results in Patients Recovered From COVID-19

Preliminary support for a 'dry swab, extraction free' protocol for SARS-CoV-2 testing via RT-qPCR

Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples

Rapid colorimetric detection of COVID-19 coronavirus using a reverse tran-scriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic plat-form: iLACO

Rapid diagnosis of SARS-CoV-2 infection by detecting IgG and IgM antibodies with an immunochromatographic device: a prospective single-center study

Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler

Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP

Reconstructed diagnostic sensitivity and specificity of the RT-PCR test for COVID-19

Report from the American Society for Microbiology COVID-19 International Summit, 23 March 2020: Value of Diagnostic Testing for SARS–CoV-2/COVID-19

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol

RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swab using Qiagen RNEasy kits or directly via omission of an extraction step

Saliva Sample as a Non-Invasive Specimen for the Diagnosis of Coronavirus Disease-2019 (COVID-19): a Cross-Sectional Study

Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs

SARS-CoV-2 detection with CRISPR diagnostics

SARS-CoV-2 Detection Using an Isothermal Amplification Reaction and a Rapid, Inexpensive Protocol for Sample Inactivation and PurificationSmart Pooled sample Testing for COVID-19: A Possible Solution for Sparsity of Test Kits

Sequential informed pooling approach to detect SARS-CoV2 infection

The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step

Variation in False Negative Rate of RT-PCR Based SARS-CoV-2 Tests by Time Since Exposure